| Autor: |
González, Celedonio, Noda, Judith, Espino, José J., Brito, Nélida |
| Předmět: |
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| Zdroj: |
Biotechnology Letters; Nov2008, Vol. 30 Issue 11, p1989-1992, 4p, 2 Black and White Photographs |
| Abstrakt: |
Current DNA extraction protocols for genomic DNA from Botrytis cinerea almost always start with mycelium that has been reduced to powder with liquid N2 in a mortar, and this makes their application to a large number of samples slow and cumbersome. Here we present an adaptation of an existing method [Möller et al. (1992) Nucleic Acids Res 20: 6115–6116] for which the initial steps have been modified, including the homogenization of the fungus with sand and the aid of a common household drill. This method allows the processing of large number of samples in much shorter times and generates an average of 4 μg DNA per sample, of sufficient quality for use in PCR and Southern blotting. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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