| Autor: |
Sjögren, Benita, Csöregh, Linda, Svenningsson, Per |
| Zdroj: |
Naunyn-Schmiedeberg's Archives of Pharmacology; Oct2008, Vol. 378 Issue 4, p441-446, 6p, 2 Graphs |
| Abstrakt: |
Lipid rafts regulate functions of various G protein-coupled receptors and signaling proteins. We show that human primary neuronal cultures contain high levels of 5-HT1A receptors. Stimulation with the 5-HT1A/7 receptor agonist, 8-OH-DPAT, reduced P-T185/Y187-ERK2. This reduction could be blocked by the 5-HT1A receptor antagonist, WAY100635. Pretreatment with the cholesterol sequestering agent, methyl-β-cyclodextrin, before adding 8-OH-DPAT, significantly counteracted the inhibitory influence of 8-OH-DPAT on P-T185/Y187-ERK2 and P-S133-CREB. These data indicate that reduction of cholesterol levels significantly influence signaling via 5-HT1A receptors in intact neurons. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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