| Autor: |
Hoey Lan Tjiong, Roel Swart, Trinet Rietveld, Josias L. Wattimena, Wim C. Hop, Marien W. Fieren, Jacobus W. van den Berg |
| Předmět: |
|
| Zdroj: |
Nephrology Dialysis Transplantation; Aug2008, Vol. 23 Issue 8, p2660-2660, 1p |
| Abstrakt: |
Background. Two well-described methods for measuring whole-body protein turnover (WBPT) are the precursor method using a primed continuous infusion of [1-13C]leucine and the end-product method with a single oral dose of [15N]glycine. We previously measured the effects of amino acid (AA)-containing dialysate on protein anabolism in patients undergoing continuous ambulatory peritoneal dialysis (CAPD) using the [1-13C]leucine technique. Here, we examine whether the less invasive [15N]glycine method could also be appropriate for studying nutritional interventions. Methods. We compared the results of WBPT measurements using a single oral dose of [15N]glycine with those obtained with the primed continuous infusion of [1-13C]leucine during AA and glucose (G) dialysis and G-only dialysis in 12 CAPD patients in the fed state. Results. The end-product method showed a wide variation for protein synthesis and breakdown measurements. It did not detect a small but significant increase in protein synthesis with AA-containing dialysate as shown by the precursor method. However, a significant relation was found between both methods for net protein synthesis (i.e. protein synthesis minus breakdown) during AA and G (r = 0.75, P = 0.005) or during G-only dialysis (r = 0.86, P < 0.001). The agreement between the two methods for the net protein balance was good [intra-class correlation coefficient (ICC) = 0.88] with G-only dialysate and moderate (ICC = 0.70) with AA and G dialysate. Conclusion. While the precursor method shows less variation, the more convenient end-product method may be useful in larger groups of selected patients including those on PD. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
