| Autor: |
Alexandre G. Dayer, Benoit Jenny, Marc-Olivier Sauvain, Gael Potter, Patrick Salmon, Eloisa Zgraggen, Michiko Kanemitsu, Eduardo Gascon, Stephane Sizonenko, Didier Trono, Jozsef Z. Kiss |
| Předmět: |
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| Zdroj: |
Brain: A Journal of Neurology; Nov2007, Vol. 130 Issue 11, p2962-2962, 1p |
| Abstrakt: |
Strategies to enhance the capacity of grafted stem/progenitors cells to generate multipotential, proliferative and migrating pools of cells in the postnatal brain could be crucial for structural repair after brain damage. We investigated whether the over-expression of basic fibroblast growth factor 2 (FGF-2) in neural progenitor cells (NPCs) could provide a robust source of migrating NPCs for tissue repair in the rat cerebral cortex. Using live imaging we provide direct evidence that FGF-2 over-expression significantly enhances the migratory capacity of grafted NPCs in complex 3D structures, such as cortical slices. Furthermore, we show that the migratory as well as proliferative properties of FGF-2 over-expressing NPCs are maintained after in vivo transplantation. Importantly, after transplantation into a neonatal ischaemic cortex, FGF-2 over-expressing NPCs efficiently invade the injured cortex and generate an increased pool of immature neurons available for brain repair. Differentiation of progenitor cells into immature neurons was correlated with a gradual down-regulation of the FGF-2 transgene. These results reveal an important role for FGF-2 in regulating NPCs functions when interacting with the host tissue and offer a potential strategy to generate a robust source of migrating and immature progenitors for repairing a neonatal ischaemic cortex. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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