| Autor: |
Dae Seok Na, Hwang Lee, Sun Uk Kim, Chang Nam Hwang, Sang Ho Lee, Ji Yoon Kang, Jai Kyeong Kim, James Jungho Pak |
| Předmět: |
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| Zdroj: |
Journal of Biomaterials Applications; Jul2008, Vol. 23 Issue 1, p73-84, 12p, 3 Black and White Photographs |
| Abstrakt: |
Various materials including glass and polymers have been widely used for stem cell culture due to their biocompatibility. However, the roles of these materials are fundamentally limited because they cannot realize or imitate the complex biological functions of living tissues, except in very simple cases. Here, the development of a bio-derived material suitable for stem cell culture and improvement of differentiation efficiency to specific cell lineages with no stimulating agents by using a chorion obtained from a fertilized zebrafish egg through the removal of the yolk and embryonic cell mass from the egg is reported. Mouse P19 EC stem cells introduced into the empty chorion form a uniform embryoid body (EB) without addition of any inducing agent. It is demonstrated that the zebrafish chorion with nanopores improves efficiencies greatly in the EB formation, cell proliferation, and lineage-specific differentiations compared to those of the conventional hanging drop culture method. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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