Selection Against Glycosylation in Ruminant Pancreatic Ribonucleases by Replacements in the Ancestral Carbohydrate Attachment Site.

Autor: Matthias Gautschi, Jaap Beintema
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Zdroj: Biochemical Genetics; Aug2008, Vol. 46 Issue 7/8, p446-450, 5p, 1 Diagram, 1 Graph
Abstrakt: Abstract  A hypothesis, proposed 25 years ago, that there is selection against glycosylation in ruminant pancreatic ribonucleases by replacement of methionine to leucine in the ancestral carbohydrate attachment site Asn–Met–Thr at residues 34–36, was experimentally confirmed. The replacement of leucine at position 35 by methionine in bovine ribonuclease resulted in a three-fold relative increase in glycosylation when expressed in Chinese hamster ovary cells. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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