| Autor: |
Bandari, Suresh, Gannu, Ramesh, Naidu, K. V. S., Yamsani, MadhusudanRao |
| Předmět: |
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| Zdroj: |
Journal of Liquid Chromatography & Related Technologies; 2008, Vol. 31 Issue 14, p2101-2112, 12p, 1 Diagram, 3 Charts, 3 Graphs |
| Abstrakt: |
A simple, specific, precise, sensitive, and rapid high performance liquid chromatographic method with UV detection was developed and validated for quantification of fenoverine in human serum. The developed method employed liquid-liquid extraction of fenoverine from serum with dichloromethane using chlorpromazine hydrochloride as internal standard (I.S). Chromatographic separation was accomplished within 10 min on an inertsil ODS column employing acetonitrile, water, and 0.375% v/v triethylamine (pH adjusted to 2.5 with orthophosphoric acid) in the ratio of 41:59 v/v at a flow rate of 1 mL min-1. Retention times of fenoverine and internal standard are 6.76 and 8.19 min, respectively. The proposed method was linear in the range of 5 to 2000 ng mL-1, specific and sensitive with limit of quantification of 5 ng mL-1, better than the reported method. The intra-day and inter-day coefficient of variation of the assay method was found to be 2.30 to 2.84 and 1.01 to 2.34, respectively, with high accuracy and precision results. This validated method was applied successfully for pharmacokinetic study of fenoverine in human serum. Such a method would be ideally suitable for the estimation of the drug for pharmacokinetic studies in human volunteers after oral administration of a single or multiple dose(s) of fenoverine. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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