| Autor: |
Berezhnoy, A. E., Wainson, A. A., Kasatkina, N. N., Khokhlova, O. A., Ostrovskaya, A. S., Volodina, E. V., Baryshnikov, A. Yu., Georgiev, G. P., Larin, S. S. |
| Předmět: |
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| Zdroj: |
Molecular Biology; May2008, Vol. 42 Issue 3, p442-448, 7p, 1 Black and White Photograph, 4 Graphs |
| Abstrakt: |
γ-irradiation is commonly used to inactivate whole-cell anticancer vaccines containing viable tumor cells. To evaluate the effect of γ-irradiation on transgene expression in tumor cells, human and mouse cell lines were stably transfected with constructs expressing the granulocyte macrophage colony-stimulating factor (GM-CSF) or green fluorescent protein (GFP) gene under the control of the immediate-early CMV promoter. Irradiation of cells at 20–100 Gy caused a loss of proliferation capacity and gradual cell death, with the survival depending on the irradiation dose. G2/M cells accumulated in irradiated cultures, while the portion of S-phase cells was reduced. Surviving cells displayed activation of β-galactosidase and morphological changes associated with cell senescence. Mitochondrial dehydrogenase activity did not change with the irradiation dose. Irradiated cells retained transgene expression. Moreover, the amount of secreted GM-CSF and GFP production significantly increased after γ-irradiation, up to tenfold in cells exposed to 100 Gy. Transgene expression increased gradually and positively correlated with the total irradiation dose. The results demonstrate that γ-irradiation at 100 Gy is optimal for whole-cell anticancer vaccine inactivation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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