Identification of glucose tolerant acid active β-glucosidases from thermophilic and thermotolerant fungi.

Autor: K. Sonia, B. Chadha, A. Badhan, H. Saini, M. Bhat
Předmět:
Zdroj: World Journal of Microbiology & Biotechnology; May2008, Vol. 24 Issue 5, p599-604, 6p
Abstrakt: Abstract  Thirteen thermophilic and thermotolerant fungal cultures isolated from composting soils produced diverse β-glucosidases as indicated by zymograms of PAGE developed using 4-methylumbelliferyl-β-d-glucoside. IEF profiling revealed the presence of 28 β-glucosidases separated on the basis of their pI. Eleven of the β-glucosidases were active under acidic conditions. Two β-glucosidase isoforms, ASCβG-II of Aspergillus caespitosus and HIβG-I of Humicola insolens were resistant to inhibition by glucose and were active in the presence of 300 and 100 (mM) glucose, respectively. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index