| Autor: |
Mäkelä, Sanna M., Jauhiainen, Matti, Ala-Korpela, Mika, Metso, Jari, Lehto, Tiina M., Savolainen, Markku J., Hannuksela, Minna L. |
| Předmět: |
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| Zdroj: |
Alcoholism: Clinical & Experimental Research; Jun2008, Vol. 32 Issue 6, p991-1000, 10p, 4 Charts, 4 Graphs |
| Abstrakt: |
Background: Alcohol consumption is associated with increased serum high density lipoprotein (HDL) cholesterol levels and a decreased risk for the development of atherosclerosis. However, the effects of heavy alcohol intake on reverse cholesterol transport, one of the key anti-atherogenic processes related to HDL, are poorly known. Methods: The ability of total HDL as well as HDL2 and HDL3 subclasses to promote cholesterol efflux from 3H-cholesterol-labeled RAW 264.7 macrophages was studied among 6 heavy alcohol drinkers and 6 controls. Distribution of HDL subclasses was analyzed by 4 to 30% native gradient gels. Serum phospholipid transfer protein (PLTP) and cholesteryl ester transfer protein (CETP) activities were analyzed among several other biochemical measures. Results: Cholesterol efflux to HDL2 of heavy drinkers was 22% ( p = 0.025) higher relative to controls. The increase in HDL2 phospholipids, with a concomitant 2-fold ( p = 0.055) increase in large HDL2b particles, was associated with enhanced cholesterol efflux to HDL2. Interestingly, the cholesterol efflux to HDL3 did not differ between the 2 study groups. These findings may be partially explained by a decreased CETP activity (−26%, p = 0.037) and an increased PLTP activity (39%, p = 0.045) in heavy drinkers. Conclusions: The increased cholesterol efflux potential of HDL2 is most likely an anti-atherogenic feature linked to heavy alcohol consumption. The cholesterol efflux and HDL phospholipids also associated strongly within the whole study group ( rs = 0.910, p ≤ 0.01) suggesting a common pathway of enhanced cholesterol efflux via enlarged phospholipid-rich HDL particles. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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