| Abstrakt: |
Abstract  This study was to investigate the alterations of the α1 nicotinic acetylcholine receptor (nAChR) levels under tensile stress stimulation in maxillofacial skeletal myocytes. The skeletal muscle satellite cells from two to three days post-natal BALB/C miceâs maxillofacial muscle were collected for primary cell culture. The second passage cells in the loaded groups were subjected to cyclic tensile stress (0.5 Hz, 2000 μ strain) produced by a four point bending system for 2, 4, 6, 8, or 12 h. In the control groups, cells were cultured on similar plates and kept in the same incubator without mechanical stress loading. The examination of nAChR α1 receptor expression was performed by receptor binding of [125I] a-bungarotoxin. The nAChR α1 mRNA transcript level was analyzed by semi-quantitative RT-PCR. The result showed that the nAChR α1 receptor expression was elevated significantly in stress-stimulated group (P P [ABSTRACT FROM AUTHOR] |
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