| Autor: |
Sancho, Ana I., van Ree, Ronald, van Leeuwen, Astrid, Meulenbroek, Bert J., van de Weg, Eric W., Gilissen, Luud J. W. J., Puehringer, Helene, Laimer, Margit, Martinelli, Alessio, Zaccharini, Marzio, Vazquez-Cortés, Sonia, Fernandez-Rivas, Montserrat, Hoffmann-Sommergruber, Karin, Mills, E. N. Clare, Zuidmeer, Laurian |
| Předmět: |
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| Zdroj: |
International Archives of Allergy & Immunology; 2008, Vol. 146 Issue 1, p19-26, 8p, 2 Charts, 4 Graphs |
| Abstrakt: |
Background: Fruits are a major cause of food allergy in adults. Lipid transfer proteins (LTP) are implicated in severe allergic reactions to fruits, but little is known about LTP content in different cultivars. Objective: Determination of the levels of LTP in a wide range of apple cultivars. Methods: LTP was measured in apples from 53 cultivars grown in Italy and 35 grown in The Netherlands, using three different immunoassays: a competitive ELISA (cELISA), a sandwich ELISA (sELISA) and a RAST inhibition (RI). Selected cultivars were evaluated using the basophil histamine release test (BHR), skin prick test (SPT) and double-blind, placebo-controlled food challenge (DBPCFC). Results: LTP levels measured with the three immunoassays were significantly correlated, as judged by Pearson’s correlation (0.61 < Rp < 0.65; p < 0.0001), but differed with respect to the actual quantities: 3.4–253.2 (sELISA), 2.7–120.2 (cELISA) and 0.4–47.3 μg/g tissue (RI). Between cultivars, LTP titers varied over about a two-log range. Pilot in vitro and in vivo biological testing (BHR, SPT and DBPCFC) with selected cultivars supported the observed differences in LTP levels. Conclusions: Around 100-fold differences in LTP levels exist between apple cultivars. Whether the lowest observed levels of LTP warrant designation as hypo-allergenic requires more extensive confirmation by oral challenges. Determination of cultivar variation in LTP levels provides important information for growers and consumers. Comparison to earlier reported Mal d 1 levels in the same cultivars reveals that a designation as low allergenic does not always coincide for both allergens. Copyright © 2007 S. Karger AG, Basel [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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