Cloning and characterization of the glyceraldehyde-3-phosphate dehydrogenase gene and the use of its promoter for expression in Myrothecium gramineum, a novel expression host.

Autor:	De Maeseneire, Sofie L., Dauvrin, Thierry, Jonniaux, Jean-Luc, Van Bogaert, Inge N. A., Soetaert, Wim K., Vandamme, Erick J. M. C.
Předmět:	CLONING GENE expression MYROTHECIUM DEHYDROGENASES HOST-fungus relationships AMYLASES NUCLEOTIDE sequence PROTEINS MICROBIOLOGY
Zdroj:	FEMS Microbiology Letters; Apr2008, Vol. 281 Issue 2, p140-146, 7p, 2 Black and White Photographs, 2 Diagrams, 2 Charts
Abstrakt:	At our laboratory, research has focused on the development of Myrothecium gramineum as a novel expression host. The glyceraldehyde-3-phosphate dehydrogenase ( gpd)-promoter of M. gramineum was isolated and characterized (Genbank accession number ). In order to prove its functionality and to explore the potential of M. gramineum as a novel fungal expression host, use of this gpd-promoter for the expression of a fungal α-amylase was investigated. Myrothecium gramineum was transformed with pGPDlpAmyAO, containing the gpd-promoter followed by the amy3 encoding sequence of Aspergillus oryzae. Study of the amylase production indicated that the promoter can be successfully used for the expression of heterologous proteins in M. gramineum. To the best of our knowledge, this is the first time a homologous expression system has been described for M. gramineum. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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