| Autor: |
Johansson, P. A., Dziegielewska, K. M., Ek, C. J., Habgood, M. D., Møllgård, K., Potter, A., Schuliga, M., Saunders, N. R. |
| Předmět: |
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| Zdroj: |
Cell & Tissue Research; Nov2005, Vol. 322 Issue 3, p353-364, 12p, 5 Diagrams, 2 Charts |
| Abstrakt: |
The normal brain develops within a well-controlled stable internal “milieu” protected by specialised mechanisms referred to collectively as blood–brain barriers. A fundamental feature of this environment is the control of water flow in and out of the developing brain. Because of limited vascularisation of the immature brain, choroid plexuses, via the cerebrospinal fluid, have been proposed as the main route of fluid exchange between the blood and brain interfaces. We describe the temporal expression and appearance of aquaporin-1 (AQP1) which is important for water transfer across adult choroid plexuses. AQP1 expression was studied in rat embryos using real time reverse transcription/polymerase chain reaction. mRNA for AQP1 was present in rat brain at embryonic day 12 (E12) one day before the protein was detectable in the fourth ventricular choroid plexus (the first plexus to appear); its relative levels increased at E13-E14 when more AQP1-immunoreactive cells appeared in all plexuses. The presence of AQP1 was determined immunocytochemically in five different mammalian species (rat, mouse, human, sheep and opossum) in all four choroid plexuses from their earliest appearance. In all five species studied, the appearance of AQP1 immunoreactivity followed the same developmental sequence: the fourth, lateral and, finally, third ventricular choroid plexus. The stage of choroid plexus development when AQP1 was first detected in all five species and in all four choroid plexuses corresponded to the transition between Stages I and II. The cellular localisation of AQP1 in all choroid plexuses, as soon as it was detectable, had the characteristic apical membrane distribution previously described in the adult; a basolateral membrane localisation was also observed. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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