Enhancement of fructanohydrolase synthesis from Aspergillus niger by simultaneous in vitro induction and in vivo acid stress using sucrose ester.

Autor: Xiang-Yang Ge, He Qian, Wei-Guo Zhang
Předmět:
Zdroj: World Journal of Microbiology & Biotechnology; Jan2008, Vol. 24 Issue 1, p133-138, 6p
Abstrakt: Abstract  This study describes a novel strategy to improve the fructanohydrolase production and growth performance of Aspergillus niger by the addition of sucrose ester to the culture medium in a 3 L fermenter. It was found that in the aerobic and non-pH-controlled condition, the sucrose ester not only acted as a more favorable in vitro inducer than inulin for fructanohydrolase formation, but also significantly forced in vivo carbon and energy flux into enzyme synthesis by acid stress. Response surface methodology (RSM) was used to optimize the composition of the culture medium, hence the fermentation period was shortened (near 50%) and enzyme activity was enhanced (over 4-fold higher), respectively. The results presented here provided a novel way to improve the inducible enzyme production by simultaneous inducement and acid stress. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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