Role of ecdysone 20-monooxygenase in regulation of 20-hydroxyecdysone levels by juvenile hormone and biogenic amines in Drosophila.

Autor: Rauschenbach, Inga Yu., Gruntenko, N. E., Chentsova, N. A., Adonyeva, N. V., Alekseev, A. A.
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Zdroj: Journal of Comparative Physiology B: Biochemical, Systemic & Environmental Physiology; Jan2008, Vol. 178 Issue 1, p27-32, 6p, 1 Diagram, 3 Graphs
Abstrakt: The effects of increased levels of dopamine (feeding flies with dopamine precursor, l-dihydroxyphenylalanine) and octopamine (feeding flies with octopamine) on ecdysone 20-monooxygenase activity in young (2 days old) wild type females (the strain wt) of Drosophila virilis have been studied. l-dihydroxyphenylalanine and octopamine feeding increases ecdysone 20-monooxygenase activity by a factor of 1.6 and 1.7, respectively. Ecdysone 20-monooxygenase activity in the young (1 day old) octopamineless females of the strain Tβh nM18 , in females of the strain P845 (precursor of Tβh nM18 strain) and in wild type females ( Canton S) of Drosophila melanogaster have been measured. The absence of octopamine leads to a considerable decrease in the enzyme activity. We have also studied the effects of juvenile hormone application on ecdysone 20-monooxygenase activity in 2-day-old wt females of D. virilis and demonstrated that an increase in juvenile hormone titre leads to an increase in the enzyme activity. We discuss the supposition that ecdysone 20-monooxygenase occupies a key position in the regulation of 20-hydroxyecdysone titre under the conditions that lead to changes in juvenile hormone titre and biogenic amine levels. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index