Thymic stromal lymphopoietin secretion of synovial fibroblasts is positively and negatively regulated by Toll-like receptors/nuclear factor-κB pathway and interferon-γ/dexamethasone.

Autor: Ozawa, Tetsuro, Koyama, Kensuke, Ando, Takashi, Ohnuma, Yuko, Hatsushika, Kyosuke, Ohba, Tetsuro, Sugiyama, Hajime, Hamada, Yoshiki, Ogawa, Hideoki, Okumura, Ko, Nakao, Atsuhito
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Zdroj: Modern Rheumatology; Dec2007, Vol. 17 Issue 6, p459-463, 5p, 4 Graphs
Abstrakt: Thymic stromal lymphopoietin (TSLP) is an interleukin (IL)-7-like cytokine produced by epithelial cells and triggers dendritic cell-mediated Th2 type allergic inflammatory responses. This study investigated whether Toll-like receptor (TLR) ligands, lipopolysaccharide (LPS) and poly-IC affect TSLP production in synovial fibroblasts. Enzyme-linked immunosorbent assay showed that LPS and poly-IC upregulated TSLP production in synovial fibroblasts obtained from patients with rheumatoid arthritis (RA) and osteoarthritis (OA). In addition, we found that nuclear factor (NF)-κB inhibitor IMD-0354, dexamethasone, and interferon (IFN)-γ inhibited the LPS- and poly-IC-induced TSLP production in RA and OA synovial fibroblasts. Thus, LPS and poly-IC can upregulate TSLP via a NF-κB pathway in synovial fibroblasts, which is downregulated by dexamethasone and interferon (IFN)-γ. The current findings suggest that TSLP may be involved in the pathophysiology of inflammatory arthritis as well as allergic disease. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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