Isolation and characterization of polymorphic microsatellite loci in black sea bream (Acanthopagrus schlegeli) by cross-species amplification with six species of the Sparidae family.

Autor: Yun-Guo Liu, Ling-Xiao Liu, Zhen-Xing Wu, Hong Lin, Ba-Fang Li, Xiu-Qin Sun
Předmět:
Zdroj: Aquatic Living Resources; Jul2007, Vol. 20 Issue 3, p257-262, 6p
Abstrakt: We tested cross-species amplification of 68 existing microsatellite loci in 6 species of the Sparidae family: Acanthopagrus butcheri, Sparus aurata, Pagrus auratus, Chrysophrys major, Pagellus bogaraveo, Pagellus erythrinus and one species of Bothidae, Paralichthys olivaceus. Of the 68 loci screened, sixteen were found to be polymorphic when tested in 20 individual black sea bream, Acanthopagrus schlegeli. The number of alleles per locus ranged from 2 to 9, and the observed and expected heterozygosity ranged from 0.55 to 0.95, and from 0.58 to 0.87, respectively. Our results show that cross-species amplification of known microsatellite loci in closely related species is a highly promising source of microsatellite markers for A. schlegeli. Nous testons l'amplification intersp?cifique de 68?locus de microsatellites existants de six esp?ces de Sparid?s : Acanthopagrus butcheri, Sparus aurata, Pagrus auratus, Chrysophrys major, Pagellus bogaraveo, Pagellus erythrinus et une esp?ce de Bothid?, Paralichthys olivaceus. Des 68?locus ?tudi?s, seize sont polymorphes lorsqu'ils sont test?s chez 20 individus de Acanthopagrus schlegeli. Le nombre d'all?les par locus varie de 2 ? 9, et l'h?t?rozygosit? (la diversit? g?n?tique) s'?tend de 0,55 ? 0,95, et de 0,58 ? 0,87, respectivement. Nos r?sultats montrent que l'amplification intersp?cifique de locus de microsatellites chez des esp?ces proches est une source prometteuse de marqueurs de microsatellites pour A. schlegeli. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index