Development of efficient method for purified recombinant bovine granulocyte-macrophage colony-stimulating factor production with baculovirus-silkworm gene expression system.

Autor:	Nagaya, Hidekazu, Kanaya, Toshimichi, Tobita, Yoneko, Yokomizo, Yuichi, Inumaru, Shigeki, Onodera, Takashi
Předmět:	GENE expression GRANULOCYTE-macrophage colony-stimulating factor COLONY-stimulating factors (Physiology) CHROMATOGRAPHIC analysis ANALYTICAL chemistry SILICA gel COLLOIDS ION exchange (Chemistry) EXCHANGE reactions
Zdroj:	Biotechnology Letters; Jan2008, Vol. 30 Issue 1, p41-45, 5p, 2 Black and White Photographs, 1 Diagram, 2 Charts
Abstrakt:	Recombinant bovine granulocyte-macrophage colony-stimulating factor (rboGM-CSF) was produced by the baculovirus-silkworm expression system. It was purified to 98% by (NH4)2SO4, followed by a three-step column chromatography with silica gel, ion exchange resin and a metal chelate column. The specific activity of purified rboGM-CSF was 1.6–6.3 × 106 ED50 mg−1. By this method, the specific activity was raised 160-fold and 21% of the expressed rboGM-CSF was recovered. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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