| Autor: |
Bergmann, Olaf, Bhardwaj, Ratan D., Barnabé-Heider, Fanie, Zdunek, Sofia, Buchholz, Bruce A., Zupicich, Joel, Druid, Henrik, Frisen, Jonas |
| Předmět: |
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| Zdroj: |
Regenerative Medicine; Nov2007 Supplement, Vol. 2, pS32-S32, 1/3p |
| Abstrakt: |
The ability of myocardial cells to regenerate in the adult mammalian heart has been a subject of controversy over the past decades. Originally, the heart was viewed as an organ that lost its ability to regenerate. However, this prevailing opinion has needed reconsideration recently in light of studies indicating that some degree of heart muscle cell turnover was indeed present under normal as well as under pathologic disease conditions. Traditional methods used for dating cells are limited or not appropriate for human use. We have taken advantage of the integration of 14C (radiocarbon), generated by nuclear bomb tests during the Cold War, into DNA in order to establish the age of cardiomyocytes in the human heart. Using flow cytometry, the nuclei of both cardiomyocyte and non-cardiomyocyte populations were isolated and the purified DNA was subjected to 14C analysis. We will present preliminary data on turnover in human cardiomyocytes. Turnover kinetics and DNA synthesis of human cardiomyocytes will be discussed. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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