| Autor: |
Henk D.F.H. Schallig, Eduardo S. da Silva, Wendy F. van der Meide, Gerard J. Schoone, Celia M.F. Gontijo |
| Předmět: |
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| Zdroj: |
Vector-Borne & Zoonotic Diseases; Sep2007, Vol. 7 Issue 3, p387-393, 7p |
| Abstrakt: |
Identification of the zoonotic reservoir is important for leishmaniasis control program. A number of (wild) animal species may serve as reservoir hosts, including the opossum Didelphis marsupialis. A survey carried out in Didelphisspecimens (n 111) from the metropolitan region of Belo Horizonte, an important focus of human leishmaniasis in Brazil, is reported. All animals were serologically tested with indirect fluorescence antibody test (IFAT) and direct agglutination tests (DAT) based on L. (L.) donovanior L. (V.) braziliensisantigen. A sub-population (n 20) was analyzed with polymerase chain reaction (PCR) for the presence of Leishmania-specific DNA. For species identification, PCR-positive samples were subjected to restriction enzyme fragment polymorphism (RFLP) analysis. Depending on the sero-diagnostic test employed, the sero-prevalence varied between 8.1 (9111 animals positive with DAT test based on L. braziliensisantigen) and 21.6 (24111 animals positive with IFAT). Five out of 20 samples analyzed with PCR tested positive for the presence of Leishmania-specific DNA. RFLP analysis revealed that two samples contained L. braziliensiscomplex DNA, one contained L. donovanicomplex DNA, and two samples could not be typed with the methodology used. These data suggest a potential role for the opossum as a reservoir host for zoonotic leishmaniasis in the region. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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