| Autor: |
Sung-Jo Kim, Eun-Young Choi, Eun Gyung Kim, Su-Hwa Shin, Ju-Youn Lee, Jeom-Il Choi, In-Soon Choi |
| Předmět: |
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| Zdroj: |
FEMS Immunology & Medical Microbiology; Nov2007, Vol. 51 Issue 2, p407-413, 7p, 3 Black and White Photographs, 2 Graphs |
| Abstrakt: |
The purpose of this study was to investigate the effects of lipopolysaccharide from Prevotella intermedia, a major cause of inflammatory periodontal disease, on the production of tumor necrosis factor (TNF)-α and the expression of TNF-α mRNA in differentiated THP-1 cells, a human monocytic cell line. The potential involvement of the three main mitogen-activated protein kinase (MAPK) signaling pathways in the induction of TNF-α production was also investigated. Lipopolysaccharide from P. intermedia ATCC 25611 was prepared by the standard hot phenol–water method. THP-1 cells were incubated in the medium supplemented with phorbol myristate acetate to induce differentiation into macrophage-like cells. It was found that P. intermedia lipopolysaccharide can induce TNF-α mRNA expression and stimulate the release of TNF-α in differentiated THP-1 cells without additional stimuli. Treatment of the cells with P. intermedia lipopolysaccharide resulted in a simultaneous activation of three MAPKs [extracellular signal-related kinase 1/2 (ERK1/2), c-Jun N-terminal kinase 1/2 (JNK1/2) and p38]. Pretreatment of the cells with MAPK inhibitors effectively suppressed P. intermedia lipopolysaccharide-induced TNF-α production without affecting the expression of TNF-α mRNA. These data thus provided good evidence that the MAPK signaling pathways are required for the regulation of P. intermedia lipopolysaccharide-induced TNF-α synthesis at the level of translation more than at the transcriptional level. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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