| Autor: |
Dumaual, Carmen, Xin Miao, Daly, Thomas M., Bruckner, Carsten, Njau, Reuben, Dong-Jing Fu, Close-Kirkwood, Sandra, Bauer, Nancy, Watanabe, Nancy, Hardenbol, Paul, Hockett, Richard D. |
| Předmět: |
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| Zdroj: |
Pharmacogenomics; Mar2007, Vol. 8 Issue 3, p293-305, 13p |
| Abstrakt: |
The combined effects of multiple polymorphisms in several drug-metabolizing enzyme and transporter genes can contribute to considerable interindividual variation in drug disposition and response. Therefore, it has been of increasing interest to generate scalable, flexible and cost-effective technologies for large-scale genotyping of the drug-metabolizing enzyme and transporter genes. However, the number of drug-metabolizing enzyme and transporter gene variants exceeds the capacity of current technologies to comprehensively assess multiple polymorphisms in a single, multiplexed assay. The Targeted Genotyping System (Affymetrix®, CA, USA) provides a solution to this challenge, by combining molecular inversion probe technology with universal microarrays to provide a method that is capable of analyzing thousands of variants in a single reaction, while remaining relatively insensitive to cross-reactivity between reaction components. This review will focus on the Targeted Genotyping System and how this technology was adapted to enable comprehensive analysis of drug-metabolizing enzyme and transporter gene polymorphisms. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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