Human urine: Epicatechin metabolites and antioxidant activity after cocoa beverage intake.

Autor: Roura, Elena, Almajano, Ma Pilar, Bilbao, Ma Lourdes Mata, Andrés-Lacueva, Cristina, Estruch, Ramon, Lamuela-Raventós, Rosa Ma
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Zdroj: Free Radical Research; Aug2007, Vol. 41 Issue 8, p943-949, 7p, 1 Chart, 1 Graph
Abstrakt: Associations between cocoa consumption in humans, excreted metabolites and total antioxidant capacity (TAC) have been scarcely investigated. The aims of the study were to investigate the epicatechin (( - )-Ec) metabolites excreted in urine samples after an intake of 40 g of cocoa powder along with the TAC of these urine samples and the relation between both the analyses. Each of the 21 volunteers received two interventions, one with a polyphenol-rich food (PRF) and one with a polyphenol-free food (PFF) in a randomized cross-over study. Urine samples were taken before and during 24 h at 0-6, 6-12 and 12-24 h periods after test intake. The excreted ( - )-Ec metabolites and the TAC were determined in urine samples by LC-MS/MS and TEAC assay, respectively. The maximum excretion of ( - )-Ec metabolites and the maximum TAC value were observed in urine samples excreted between 6 and 12 h after PRF consumption. Significance of TAC increase was found in urine samples excreted during 0-6 and 6-12 h (66.6 and 72.67%, respectively, with respect to the 0 h). [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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