| Autor: |
di Gennaro, Patrizia, Ferrara, Silvia, Ronco, Ilaria, Galli, Enrica, Sello, Guido, Papacchini, Maddalena, Bestetti, Giuseppina |
| Předmět: |
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| Zdroj: |
Archives of Microbiology; Aug2007, Vol. 188 Issue 2, p117-125, 9p, 2 Color Photographs, 2 Diagrams, 2 Charts, 1 Graph |
| Abstrakt: |
Pseudomonas fluorescens ST is a styrene degrading microorganism that, by the sequential oxidation of the vinyl side chain, converts styrene to phenylacetic acid. The cluster of styrene upper pathway catabolic genes ( sty genes) has been previously localized on a chromosomal region. This report describes the isolation, sequencing and analysis of a new chromosomal fragment deriving from the ST strain genomic bank that contains the styrene lower degradative pathway genes ( paa genes), involved in the metabolism of phenylacetic acid. Analysis of the paa gene cluster led to the description of 14 putative genes: a gene encoding a phenylacetyl-CoA ligase ( paaF), the enzyme required for the activation of phenylacetic acid; five ORFs encoding the subunits of a ring hydroxylation multienzymatic system ( paaGHIJK); the gene paaW encoding a membrane protein of unknown function; five genes for a β-oxidation-like system ( paaABCDE), involved in the steps following the aromatic ring cleavage; a gene encoding a putative permease ( paaL) and a gene ( paaN) probably involved in the aromatic ring cleavage. The function of some of the isolated genes has been proved by means of biotransformation experiments. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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