| Autor: |
Murfee, Walter L., Rappleye, Jeff W., Schmid-Schönbein, Geert W. |
| Předmět: |
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| Zdroj: |
FASEB Journal; Apr2007, Vol. 21 Issue 5, pA490-A490, 1/5p |
| Abstrakt: |
Lymphatic fluid uptake from the interstitium requires the primary valve system located at endothelial cell junctions. However, the exact structure of this valve system is not well established. The objective of this study was to characterize the junctional structure of endothelial cells along the hierarchy of an intact adult initial lymphatic network. Mesenteric tissues from adult male Wistar rats were labeled with antibodies against PECAM-1 and VE-cadherin. Endothelial cells along initial lymphatics and blood microvascular networks expressed both junctional molecules. PECAM and VE-cadherin labeling patterns were continuous along vascular endothelial cell junctions, but discontinuous with gaps along lymphatic endothelial cell junctions. Along larger draining vessels in proximal regions of the initial lymphatic network, the majority of labeling gaps along junctions were less than 3 µm. In comparison to draining vessels, terminal lymphatics exhibited a decrease in PECAM staining intensity and a decrease in endothelial cell junctional length defined by positive PECAM and VE-cadherin staining. These results suggest that initial lymphatics have a discontinuous expression pattern of endothelial junction molecules facilitating the ability to separate local membrane regions between neighboring endothelial cells and forming primary valve structures. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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