| Autor: |
De Aquino Freitas, Raphael Ribeiro, Ribeiro Barreto, Carolina, Barbosa De Oliveira-Sales, Elizabeth, Aparecida Boim, Miriam, Toledo Bergamaschi, Cassia, Sterman Dolnikoff, Miriam, Ribeiro De Campos, Ruy |
| Předmět: |
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| Zdroj: |
FASEB Journal; Apr2007, Vol. 21 Issue 6, pA1195-A1195, 1/4p |
| Abstrakt: |
There is a relationship between sucrose feeding and arterial hypertension. Dysregulation of sodium and/or water reabsorption by the kidney may contribute to hypertension. The objective of this study was to investigate, in rats with overload of sucrose in diet, the effect on creatinine clearance (CCl), fractional excretion of Na+ (FENa), fractional excretion of K+ (FEK) and alterations in the mRNA expression levels of the kidney. Wistar rats (150-180g) were treated for 30 days with sucrose solution (20%) instead of drink water (SC; n=7), the control group (C; n=8) had tap water ad libitum. Mean arterial pressure (MAP), were recorded "on line" in a Power Lab system. The mRNA expression levels of epithelial sodium channel (ENaC) and water channel protein (AQP2) were analyzed in the cortical kidney tissue by Real Time RT-PCR technique. A significant (P<0.05) increase in MAP was observed in SC (128±1mmHg) compared with C (10±4 mmHg). The following values for renal function parameters were obtained for the sucrose and control groups: urinary sodium 77.6 ± 11.4 vs 144.7 ± 9.4 mEq/L (P<0.05); CCl 1.57 ± 0.22 vs 0.93 ± 0.07 ml/min-1 (P < 0.05); FENa 0.7 ± 0.10 vs 0.98 ± 0,07% (P < 0.05); FEK 36.4 ± 7.8 vs 31.4 ± 3.1%. The sucrose group showed significant increase in ENaC expression and AQP2 expression when compared with control group (SC: 1.95 vs. C: 1.03 and SC: 2.12 vs C: 1.07 respectively, P<0.05). Taken altogether, the results suggest that a decreased of FENa and an increased of mRNA expression level of ENaC and AQP2 may lead to arterial hypertension due to an augment of Na+ and water reabsorption. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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