| Autor: |
Shu Zhang, Knight, Travis J., Reecy, James M., Trenkle, Allen H., Beitz, Donald C. |
| Předmět: |
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| Zdroj: |
FASEB Journal; Apr2007, Vol. 21 Issue 6, pA1120-A1120, 1p |
| Abstrakt: |
The objective of this study was to identify single nucleotide polymorphisms (SNPs) in bovine fatty acid synthase (FAS) gene and to test the association of SNPs with beef fatty acid composition. Resequencing of the four exons of the thioesterase (TE) domain of FAS from Angus bulls identified three novel SNPs, A17924G, C18663T, and C18727T. Polymorphism A17924G was predicted to result in an amino acid change from threonine (genotype AA) to alanine (genotype GG). A PCR-RFLP assay was developed to genotype 345 Angus bulls for the A17924G SNP in which three genotype classes were detected, AA (n=126), AG (n = 177), and GG (n = 42). The genotypes were significantly associated with fatty acid composition of longissimus dorsi (LD) muscle of purebred Angus bulls. Cattle with the GG genotype had a lower atherogenic index (AI), less percentages of myristic acid (14:0) and total saturated fatty acid (SFA), and greater contents of oleic acid (18:1) and total monounsaturated fatty acids (MUFA) in the triacylglycerol (TAG) fraction of LD muscle than did the beef cattle with genotypes AA and AG, respectively (P < 0.05). The differences of individual fatty acid percentage in total lipid of LD muscle were similar as those in TAG fraction. In conclusion, this SNP may be used as a DNA marker to select breeding stock to improve the healthfulness of fatty acids in beef. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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