| Autor: |
Hazelton, Sarah Rodriguez, Spurlock, Diane Moody, Bidwell, Christopher A., Koser, Stephanie L., Donkin, Shawn S. |
| Předmět: |
|
| Zdroj: |
FASEB Journal; Apr2007, Vol. 21 Issue 6, pA1106-A1106, 1p |
| Abstrakt: |
Pyruvate carboxylase (PC) catalyzes a pivotal reaction in gluconeogenesis and lipid metabolism. We identified six unique alternative variants in the 5′ untranslated region (UTR) of bovine PC mRNA. The objectives for this study were to determine the intron and exon organization of the UTR for the bovine PC gene, to identify PC promoter regions, and to determine effects of dexamethasone (DEX) on PC promoter activity. Two BAC clones were screened with oligonucleotide sequences for UTR and coding sequence of bovine PC, isolated, and sequenced. The genomic sequence indicates the exon arrangement: 1 (48 bp), II (41 bp), IIIA (110 bp), IIIB (68 bp) and IV (185 bp) that contains 3 promoters located adjacent to exon I (P48), II (P41) and IIIA (P110). Each promoter was cloned into a firefly luciferase vector and transfected into H4IIE cells and luciferase activity for each was comparable to the minimal promoter luciferase vector (P < 0.01) but greater than the promoterless vector (P < 0.01). Promoter P110 exhibited greater luciferase activity compared with promoter P48 or P41 (P < 0.01). In stable cells lines treated with 100 nM DEX for 1, 6, 12 or 24 h the activity of P41 but not promoter P48 was increased. These data document the exon arrangement of UTR, identify the promoter regions for the bovine PC gene and indicate glucocorticoid responsiveness for promoter P41 of the bovine PC gene. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Plný text