| Autor: |
XiYong Yu, XiaoHong Li, QiuXiong Lin, ZhiXin Shan, GuangFeng Zhang, Xiao Zhang, Min Yang, ShuGuang Lin |
| Předmět: |
|
| Zdroj: |
FASEB Journal; Apr2007, Vol. 21 Issue 6, pA803-A803, 1/4p |
| Abstrakt: |
To investigate the mechanisms of bone marrow derived mesenchymal stem cell (BMSCs) differentiation induced by mimic myocardial microenvironment. BMSCs were isolated from human, and myocardial cells obtained from neonatal rat ventricles. BMSCs were cocultured with rat myocytes using semipermeable membrane. The differentiation of hBMSCs was confirmed by real-time PCR, morphologic analysis, western blot, and contractile properties. Gene chips were used to compare the gene expression profiles of BMSCs with or without induction. After cocultured with rat myocytes, the first contractions from BMSCs were noted at day 7. Some BMSCs became hGATA-4, sarcomeric α-actinin and cTnI positive. However, the c-kit, a stem cell marker, was expressed only before BMSCs induction. There were 511 genes upregulated and 295 genes downregulated after 2 weeks induction of BMSCs in cardiomyocytes microenvironment. Gene expression analysis revealed a significant contribution of Wnt pathway, Ca2+ transport, and antiapoptosis genes in the process of BMSCs differentiation. These results showed that BMSCs possesses the differentiation potential to cardiomyocyte in suitable microenvironment. The identification of gene regulating and signaling pathway will lead to a better understanding of BMSCs' oriented differentiation and molecular targets in myocardial regeneration. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Plný text