| Autor: |
Risher, J. Devon, Rivenbark, Ashley G., Coleman, William B. |
| Předmět: |
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| Zdroj: |
FASEB Journal; Apr2007, Vol. 21 Issue 6, pA756-A756, 1/5p |
| Abstrakt: |
A group of epigenetically-regulated genes (n=36) reported to be methylated in human cancer was examined for differential expression and promoter methylation in a panel of human breast cancer cell lines (n=12), revealing two groups with distinct methylation signatures. Cell lines of the hypermethylator group (n=7) exhibit concordant methylation and gene silencing of numerous epigenetically-regulated genes, while cells of the normal methylator group (n=5) lack expression of fewer of these genes. The hypermethylator phenotype is accurately predicted by loss of CST6, SCNNIA, ESR1, and CDH1 (correct assignments 75-92%), but methylation-dependent silencing of CEACAM6 correctly predicts methylator status, in 100% of cell lines. When these five genes are considered together, hypermethylator cell lines lack expression of a great majority (91% ± 4%) while normal methylator cell lines lack expression of only 20% (± 6%, p<0.0001). These findings strongly suggest the presence of a hypermethylator phenotype in human breast cancer cells which is predicted by CEACAM6 promoter methylation. CEACAM6-negative cell lines exhibit.concordant hypermethylation of other epigenetically-regulated genes and are expected to exhibit aggressive cellular characteristics. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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