| Autor: |
Murcia, Martha I., García, María J., Otal, Isabel, Gómez, Ana. B., Menéndez, M. Carmen |
| Předmět: |
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| Zdroj: |
FEMS Microbiology Letters; Jul2007, Vol. 272 Issue 2, p229-237, 9p, 1 Black and White Photograph, 2 Diagrams, 2 Charts |
| Abstrakt: |
Human clinical isolates of the Mycobacterium avium complex, from hospitals in Bogotá, were studied using a wide range of molecular tests including PCR restriction-enzyme analysis (PRA) of the hsp65 gene. Up to 21 of the isolates were identified as M. avium PRA variant III (Mav III), a variant obtained only from isolates on the American continent. In contrast to previous reports, restriction fragment length polymorphism analysis using IS 1245 and IS 1311 showed a single copy for each insertion sequence (IS) in the majority (19/21) of the Colombian Mav III isolates under study. In order to analyse whether the ISs were inserted in a relevant genomic region, experimental conditions were established to determine the insertion loci of each single copy of both ISs in the genome. Analysis of genomic insertion loci indicated that both IS 1245 and IS 1311 were present in areas containing putatively truncated integrases and/or transposases, which may have an influence on the mobility of the inserted IS. In addition, a conserved genomic region was identified for the insertion of IS 1311; this region could be part of the IS 1311 itself. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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