Role of Calcium in Exocytosis Induced by Hypotonic Swelling.

Autor:	FEDOROVICH, SERGEI V., WASEEM, TATYANA V., LAVRUKEVICH, TATYANA V., KONEV, SERGEI V.
Předmět:	CALCIUM HIGH-calcium diet BODY fluid disorders EXOCYTOSIS CELL physiology
Zdroj:	Annals of the New York Academy of Sciences; 2005, Vol. 1048 Issue 1, p337-340, 4p
Abstrakt:	We studied the influence of Ca2+ on exocytosis induced by hypotonic shock using the fluorescent dyes acridine orange FM1-43 and FM2-10. It was shown using acridine orange that lowering osmolarity to 230 mOsm/L induces exocytosis both in calcium-containing and calcium-free media. By contrast, we were able to demonstrate calcium dependence of exocytosis using styryl dyes. Lowering osmolarity leads to an increase of neurotransmitter release in a calcium-independent manner. Thus, our data suggest that hypotonic swelling induces calcium-independent exocytosis. Calcium influx mediated by stretch channels is able to switch mode of exocytosis from "kiss and run" to full fusion. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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