Isolation of Nucleic Acid Binding Proteins: An Approach for Isolation of Cell Surface, Nucleic Acid Binding Proteins.

Autor: CHELOBANOV, BORIS P., LAKTIONOV, PAVEL P., KHARKOVA, MARIA V., RYKOVA, ELENA Y., VLASSOV, VALENTIN V.
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Zdroj: Annals of the New York Academy of Sciences; 2004, Vol. 1022 Issue 1, p239-243, 5p
Abstrakt: An approach for Isolation of cell surface, nucleic acid binding proteins is described. This approach relies on affinity modification of the proteins of living cells with reactive oligonucleotides bearing a haptenic group. Covalently modified proteins were isolated by hapten-specific affinity chromatography with subsequent SDS-PAGE. Isolated 68-kDa proteins responsible for the binding of oligonucleotides were MS/MS sequenced and Identified as keratin K!, keratin Kb, keratin K2e, and albumin. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index