Autor: |
CHELOBANOV, BORIS P., LAKTIONOV, PAVEL P., KHARKOVA, MARIA V., RYKOVA, ELENA Y., VLASSOV, VALENTIN V. |
Předmět: |
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Zdroj: |
Annals of the New York Academy of Sciences; 2004, Vol. 1022 Issue 1, p239-243, 5p |
Abstrakt: |
An approach for Isolation of cell surface, nucleic acid binding proteins is described. This approach relies on affinity modification of the proteins of living cells with reactive oligonucleotides bearing a haptenic group. Covalently modified proteins were isolated by hapten-specific affinity chromatography with subsequent SDS-PAGE. Isolated 68-kDa proteins responsible for the binding of oligonucleotides were MS/MS sequenced and Identified as keratin K!, keratin Kb, keratin K2e, and albumin. [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
Externí odkaz: |
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