| Autor: |
Ponkratov, V. V., Wiedersich, J., Friedrich, J., Vanderkooi, J. M. |
| Předmět: |
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| Zdroj: |
Journal of Chemical Physics; 4/28/2007, Vol. 126 Issue 16, p165104, 5p, 4 Graphs |
| Abstrakt: |
The authors compared the spectral response of Zn-substituted horseradish peroxidase in a glycerol/water solvent to hydrostatic pressure at 2 K and ambient temperature. The low temperature experiments clearly demonstrate the presence of at least three different conformations with drastically different elastic properties. However, the main conformation, which determines the fluorescence spectrum at ambient temperature, did not show any significant difference between low and high temperature and pressure. The authors conclude that the local compressibility of the heme pocket of the protein depends only very weakly on temperature. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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