| Autor: |
W. Morelle, J-C. Michalsky, E. Dei-Cas |
| Předmět: |
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| Zdroj: |
Parasitology Research; Mar2007, Vol. 100 Issue 4, p715-720, 6p |
| Abstrakt: |
Abstract??In the present work, glycoproteins in the excretory/secretory products ofG. intestinaliswere identified and the reactivity in serum of immunized mice with these molecules was evaluated by western blotting before and after chemical treatment or enzymatic deglycosylation. Glycoproteins of 58 and 63?kDa were revealed in E/S products after periodic acid-Schiff (PAS) stain. Studies of carbohydrate specificity using digoxigenin-labeled lectins, revealed the presence ofO-glycans andN-glycans. Chemical treatment of excretory/secretory products with sodium meta-periodate or enzymatic deglycosylation withN-glycosidase F reduced the reactivity in serum for proteins of 36, 58 and 63?kDa, respectively. These results show the presence of glycoproteins in E/S products ofG. intestinalisand suggest that the antibody response is directed against glycoepitopes. The expression of carbohydrate moieties in the E/S-G. intestinalismay play an essential role in the antibody response and may be a target for serodiagnosis or immune intervention in human giardiasis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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