| Autor: |
Biget, Laurent, McCarthy, Andrew A., Lin, Chenwei, Petiard, Vincent, Tanksley, Steve D., McCarthy, James G. |
| Předmět: |
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| Zdroj: |
Acta Crystallographica: Section F (Wiley-Blackwell); Apr2007, Vol. 63 Issue 4, p304-307, 4p, 1 Color Photograph, 2 Black and White Photographs, 1 Chart |
| Abstrakt: |
Caffeine is a secondary metabolite produced by a variety of plants including Coffea canephora ( robusta) and there is growing evidence that caffeine is part of a chemical defence strategy protecting young leaves and seeds from potential predators. The genes encoding XMT and DXMT, the enzymes from Coffea canephora ( robusta) that catalyse the three independent N-methyl transfer reactions in the caffeine-biosynthesis pathway, have been cloned and the proteins have been expressed in Escherichia coli. Both proteins have been crystallized in the presence of the demethylated cofactor S-adenosyl-l-cysteine (SAH) and substrate (xanthosine for XMT and theobromine for DXMT). The crystals are orthorhombic, with space group P212121 for XMT and C2221 for DXMT. X-ray diffraction to 2.8 Å for XMT and to 2.5 Å for DXMT have been collected on beamline ID23-1 at the ESRF. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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