| Autor: |
Tajik, N., Salari, F., Hajilooi, M., Amoli, M., Salekmoghaddam, A. |
| Předmět: |
|
| Zdroj: |
Tissue Antigens; Apr2007, Vol. 69 Issue 4, p338-341, 4p, 2 Diagrams, 2 Charts |
| Abstrakt: |
Intercellular adhesion molecule 1 (ICAM-1) is a cell surface glycoprotein member of the immunoglobulin superfamily and is actively involved in immune and inflammatory responses. We introduce a novel polymerase chain reaction–sequence-specific primers (PCR–SSP) method for rapid and simultaneous genotyping of ICAM-1 G241R and K469E polymorphisms. In a total of 184 DNA samples that have been previously analyzed for these polymorphisms using polymerase chain reaction–restriction fragment length polymorphism technique, re-genotyping of all samples with this new assay showed accurate and reproducible results. As PCR–SSP-based genotyping protocols are more convenient and cost-effective to do, it could therefore offer a valuable tool for assessment of ICAM-1 polymorphisms to which more confirmatory studies are needed. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
