L-ARGININE-DEPENDENT NO SYNTHESIS AND D-GLUCOSE UPTAKE IN RAT IMMUNE CELLS FOLLOWING MUSCLE COMPRESSION INJURY AND TREATMENT WITH THE NEWLY SYNTHESIZED Nα-PARA-BUTOXYBENZOYL-LARGININE SALTS.

Autor: Alchujyan, N. Kh., Movsesyan, N. H., Kevorkian, G. A., Movsesyan, H. A., Arakelyan, L. N., Ghazaryan, S. H., Grigoryan, K. P., Sorenson, J. R. J., Greenaway, F. T.
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Zdroj: Electronic Journal of Natural Sciences; Jan2007, Vol. 8 Issue 1, p6-16, 11p, 1 Diagram, 3 Charts, 1 Graph
Abstrakt: Newly synthesized inhibitors of nitric oxide synthase (NOS) activity sodium Nα-para-butoxybenzoyl-L-argininate (NaNPBA) and lithium Nα-para-butoxybenzoyl-L-argininate (LiNPBA) were examined following treatment of rats with regard to their ability to modify activity the calcium-calmodulin-independent "inducible" NOS (iNOS) and D-glucose uptake in rat leukocytes: tissue-derived thymocytes and splenocytes as well as blood-derived lymphocytes, monocytes, and neutrophils prior to compression injury (CI) and 2 and 24 h following CI. These examinations were performed using the Griess-Ilosvay reaction to measure the level of nitrogene oxides (NOx) and related nitrosocompounds (NC) as well as L-arginine-dependent production of NOx and NC during long-term incubation of mentioned cells isolated from rats treated with 0,9% NaCL (saline) (control rats), NaNPBA, or LiNPBA. Both NaNPBA and LiNPBA modified iNOS produced NOx and related NC however the pattern of these changes varied based upon cell type when compared to cells obtained form saline-treated control rats either prior to or following CI. D-glucose uptake by these cells was also affected by NaNPBA and LiNPBA treatments and the pattern of changes in D-glucose uptake also varied based upon cell type when compared to cells obtained from saline-treated control rats prior to and following CI. It is concluded that the iNOS activity, level of NOx and related NC and D-glucose absorbance of rat immune cells are linked and contribute to pathophysiological changes in CI and are amenable to the effect of novel Nα-substituted arginine derivative salts, NaNPBA and LiNPBA in early stages of CI. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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