| Autor: |
Kusano, Nario, Hirashima, Keita, Kuwahara, Minoru, Narahara, Kenji, Imamura, Tadashi, Mimori, Tomohiro, Nakahira, Ken, Torii, Kuniaki |
| Předmět: |
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| Zdroj: |
Journal of General Plant Pathology; Feb2007, Vol. 73 Issue 1, p66-71, 6p, 2 Color Photographs, 2 Diagrams, 4 Charts |
| Abstrakt: |
A simple and rapid immunochromatographic assay (ICA) to detect Satsuma dwarf virus (SDV) was developed using colloidal gold conjugates of anti-SDV monoclonal antibodies. Of six homogenization buffers tested, 0.1 M citrate buffer (pH 7.0) gave the best results for the ICA. In the ICA, addition of 0.1% thioglycolic acid in the homogenization buffers that have been widely used in enzyme-linked immunosorbent assays (ELISA) was deleterious to the reaction because of undesirable coagulation of the colloidal gold. ICA using the anti-SDV monoclonal antibodies was 8 times and 16 times more sensitive than double antibody sandwich-ELISA and ICA using the anti-SDV polyclonal antibody, respectively. The analysis is complete in only 15 min. Furthermore, ICA using the anti-SDV monoclonal antibodies could also detect SDV-related viruses. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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