| Autor: |
Jaecheon Lee, Yunjaie Choi, Peter-Changwhan Lee, Seungha Kang, Jinduck Bok, Jaiesoon Cho |
| Předmět: |
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| Zdroj: |
World Journal of Microbiology & Biotechnology; Mar2007, Vol. 23 Issue 3, p443-446, 4p |
| Abstrakt: |
Abstract??The 1,332?bp phytase gene ofPenicilliumoxalicumPJ3 was inserted into the expression vector, pPICZ?A and expressed in the methylotrophic yeast,Pichia pastorisas an active, extracellular phytase. The recombinant phytase reached a maximum yield of 12?U/ml of medium at 120?h of cultivation after methanol induction under shake-flask conditions. The enzyme was glycosylated, with a molecular mass of about 62.5?kDa. The Michaelis constant (Km) and maximum reaction rate (Vmax) for sodium phytate was 0.37?mM and 526.3?U/mg of protein, respectively. The optimal activity occurred at pH 4.5 and 55?C. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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