| Abstrakt: |
Abstract??The CW-cytoplasmic male sterility (CMS) line has the cytoplasm ofOryza rufipogonGriff, and mature pollen is morphologically normal under an optical microscope but lacks the ability to germinate; restorer geneRf17has been identified as restoring this ability. The difference between nuclear gene expression in mature anthers was compared for the CW-CMS line, [cms-CW]rf17rf17, and a maintainer line with normal cytoplasm ofOryza sativaL., [normal]rf17rf17. Using a 22-k rice oligoarray we detected 58 genes that were up-regulated more than threefold in the CW-CMS line. Expression in other organs was further investigated for 20 genes using RT-PCR. Five genes, including genes for alternative oxidase, were found to be preferentially expressed in [cms-CW]rf17rf17but not in [normal]rf17rf17or [cms-CW]Rf17Rf17. Such [cms-CW]rf17rf17-specific gene expression was only observed in mature anthers but not in leaves, stems, or roots, indicating the presence of anther-specific mitochondrial retrograde regulation of nuclear gene expression, and thatRf17has a role in restoring the ectopic gene expression. We also used a proteomic approach to discover the retrograde regulated proteins and identified six proteins that were accumulated differently. These results reveal organ-specific induced mitochondrial retrograde pathways affecting nuclear gene expression possibly related to CMS. [ABSTRACT FROM AUTHOR] |
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