| Autor: |
Baan, Carla C., Velthuis, Jurjen H. L., Van Gurp, Evelien A. F. J., Mol, Wendy M., Klepper, Mariska, Ijzermans, Jan N.M., Weimar, Willem |
| Předmět: |
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| Zdroj: |
Clinical Transplantation; Jan2007, Vol. 21 Issue 1, p63-71, 9p, 2 Charts, 3 Graphs |
| Abstrakt: |
Background: Evidence from animal studies indicate a crucial role for CD25bright+ regulatory T cells in transplantation tolerance. Methods: To assess whether peripheral CD25bright+ T cells control immune responses in immunosuppressed kidney transplant patients, we analyzed the suppressive capacities of these cells using mixed lymphocytes reactions. Results: Allogeneic stimulation of patients peripheral blood mononuclear cells was associated with IL-2 production and T-cell proliferation. Depletion of CD25bright+ T cells resulted in a 35% (median) higher IL-2 production and a 38% higher proliferative response against third party cells, showing that functional regulatory CD25bright+ T cells were present (p = 0.03 and 0.02 respectively). In eight out of 11 patients, we also demonstrated regulation activity against donor-activated T cells (p = 0.03). These data were confirmed in coculture experiments with isolated CD25−/dim T cells plus CD25bright+ T cells. At a 1:2 ratio, the CD25bright+ T cells suppressed the proliferation of CD25−/dim donor- and third party-stimulated responder T cells. Conclusions: CD25bright+ T cells with immune regulatory activities against anti-donor-responsive T cells are readily detectable in renal allograft recipients during treatment with full dosage immunosuppression. Whether CD25bright+ T cells indeed play a role in graft acceptance after organ transplantation in patients remains to be elucidated. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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