| Autor: |
de Souza Liberal, A. T., Basílio, A. C. M., do Monte Resende, A., Brasileiro, B. T. V., da Silva-Filho, E. A., de Morais, J. O. F., Simões, D. A., de Morais, Jr., M. A. |
| Předmět: |
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| Zdroj: |
Journal of Applied Microbiology; Feb2007, Vol. 102 Issue 2, p538-547, 10p, 1 Chart, 7 Graphs |
| Abstrakt: |
Aims: To identify and characterize the main contaminant yeast species detected in fuel-ethanol production plants in Northeast region of Brazil by using molecular methods. Methods and Results: Total DNA from yeast colonies isolated from the fermentation must of industrial alcohol plants was submitted to PCR fingerprinting, D1/D2 28S rDNA sequencing and species-specific PCR analysis. The most frequent non- Saccharomyces cerevisiae isolates were identified as belonging to the species Dekkera bruxellensis, and several genetic strains could be discriminated among the isolates. The yeast population dynamics was followed on a daily basis during a whole crop harvesting period in a particular industry, showing the potential of D. bruxellensis to grow faster than S. cerevisiae in industrial conditions, causing recurrent and severe contamination episodes. Conclusions: The results showed that D. bruxellensis is one of the most important contaminant yeasts in distilleries producing fuel-ethanol from crude sugar cane juice, specially in continuous fermentation systems. Significance and Impact of the Study: Severe contamination of the industrial fermentation process by Dekkera yeasts has a negative impact on ethanol yield and productivity. Therefore, early detection of D. bruxellensis in industrial musts may avoid operational problems in alcohol-producing plants. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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