| Autor: |
Jacobs, Erin C., Campagnoni, Celia, Kampf, Kathy, Reyes, Samuel D., Kalra, Vikram, Handley, Vance, Xie, Yuan‐Yun, Hong‐Hu, Yan, Spreur, Vilma, Fisher, Robin S., Campagnoni, Anthony T. |
| Předmět: |
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| Zdroj: |
European Journal of Neuroscience; Jan2007, Vol. 25 Issue 1, p17-30, 14p |
| Abstrakt: |
The first postmitotic neurons in the developing neocortex establish the preplate layer. These early-born neurons have a significant influence on the circuitry of the developing cortex. However, the exact timing and trajectory of their projections, between cortical hemispheres and intra- and extra-cortical regions, remain unresolved. Here, we describe the creation of a transgenic mouse using a 1.3 kb golli promoter element of the myelin basic protein gene to target expression of a τ–green fluorescent protein (GFP) fusion protein in the cell bodies and processes of pioneer cortical neurons. During embryonic and early neonatal development, the timing and patterning of process extension from these neurons was examined. Analysis of τ-GFP fluorescent fibers revealed that progression of early labeled projections was interrupted unexpectedly by transient pauses at the corticostriatal and telencephalic–diencephalic boundaries before invading the thalamus just prior to birth. After birth the pioneering projections differentially invaded the thalamus, excluding some nuclei, e.g. medial and lateral geniculate, until postnatal days 10–14. Early labeled projections were also found to cross to the contralateral hemisphere as well as to the superior colliculus. These results indicate that early corticothalamic projections appear to pause before invading specific subcortical regions during development, that there is developmental regulation of innervation of individual thalamic nuclei, and that these early-generated neurons also establish early projections to commissural and subcortical targets. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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