| Abstrakt: |
Abstract??In Lambar?n? (Gabon), where a high level ofPlasmodium falciparumresistance to chloroquine has been reported, we assessed the relationship between polymorphisms in theP. falciparumchloroquine resistance transporter (Pfcrt) and multidrug resistance-1 (Pfmdr1) genes and the clinical severity of malaria. Ninety-one and 60P. falciparumisolates from children with uncomplicated or severe malaria were collected in 1996 and 2002, respectively. Single nucleotide mutations at codon 76 in thePfcrtgene and at codons 86, 184, 1034, 1042, and 1246 in thePfmdr1gene were assessed by PCR-RFLP. AllP. falciparumisolates presented thePfcrtK76T mutation, whatever the clinical status. A high prevalence (>80%) of thePfmdr186Tyr and 184Phe mutations was detected at both time points and in both clinical groups. We did not identify any specific mutation in thePfmdr1gene associated with the severity of disease, and the multiplicity ofP. falciparuminfection was also similar in both groups. Our results showed no change in the polymorphism ofPfcrtandPfmdr1genes inP. falciparumisolates collected in 1996 and 2002, and the severity of the disease was not associated with specific mutations neither in thePfcrtnor in thePfmdr1genes in the study site. [ABSTRACT FROM AUTHOR] |
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