Response of CH4 oxidation and methanotrophic diversity to NH4 and CH4 mixing ratios.

Autor: Svetlana Bykova, Pascal Boeckx, Irina Kravchenko, Valery Galchenko, Oswald Van Cleemput
Předmět:
Zdroj: Biology & Fertility of Soils; Jan2007, Vol. 43 Issue 3, p341-348, 8p
Abstrakt:
Abstract??Methane oxidising activity and community structure of 11, specifically targeted, methanotrophic species have been examined in an arable soil. Soils were sampled from three different field plots, receiving no fertilisation (C), compost (G) and mineral fertiliser (M), respectively. Incubation experiments were carried out with and without pre-incubation at elevated CH4mixing ratios (100?ml CH4l?1) and with and without ammonium (100?mg N kg?1) pre-incubation. Four months after fertilisation, plots C, G and M did not show significant differences in physicochemical properties and CH4oxidising activity. The total number of methanotrophs (determined as the sum the 11 specifically targeted methanotrophs) in the fresh soils was 17.0?106, 13.7?106and 15.5?106cells g?1for treatment C, G and M, respectively. This corresponded to 0.11 to 0.32% of the total bacterial number. The CH4oxidising activity increased 105-fold (20?26?mg CH4g?1h?1), the total number of methanotrophs doubled (28?76?106cells g?1) and the methanotrophic diversity markedly increased in treatments with a pre-incubation at elevated CH4concentrations. In all soils and treatments, type II methanotrophs (62?91%) outnumbered type I methanotrophs (9?38%).MethylocystisandMethylosinusspecies were always most abundant. After pre-incubation with ammonium, CH4oxidation was completely inhibited; however, no change in the methanotrophic community structure could be detected.
[ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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