| Autor: |
Luciana C. Caperuto, Gabriel F. Anhê, Angélica M. Amanso, Luciene M. Ribeiro, Mayrin C. Medina, Lilian C. Souza, Olga M. F. Carvalho, Silvana Bordin, Mário J. A. Saad, Carla R. O. Carvalho |
| Předmět: |
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| Zdroj: |
Endocrine (1355008X); Jun2006, Vol. 29 Issue 3, p391-398, 8p |
| Abstrakt: |
In the present study, we investigated the protein levelsand phosphorylation status of the insulin receptor andinsulin receptor substrates (IRS-1, IRS-2, and IRS-3) aswell as their association with PI(3)-kinase in the ratadipose tissue of two models of insulin resistance: dexamethasonetreatment and aging. AKT and atypical PKCphosphorylation detection were also performed. Bothmodels showed decreased insulin-induced IRS-1 andIRS-2 tyrosine phosphorylation, accompanied by reducedprotein levels of IRS-1 and IRS-2. Nevertheless,IRS-3 protein level was unchanged in aging but increasedin dexamethasone-treated rats. PI(3)-kinaseassociation with IRS-1 was reduced in aged rats, whereasdexamethasone-treated rats showed a reduced IRS-2/PI(3)-kinase association. However, IRS-3 associationwith PI(3)-kinase was reduced in both models, as wellas insulin-induced AKT and PKC phosphorylation. Thealterations described in the present study show thatthe action of insulin is differently impaired dependingon the origin of insulin resistance. These differencesmight be directly linked to the singular metabolic featuresof the models we tested. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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