| Autor: |
de Champdoré, Marcella, Staiano, Maria, Aurilia, Vincenzo, Stepanenko, Olesia V., Parracino, Antonietta, Rossi, Mosè, D'Auria, Sabato |
| Předmět: |
|
| Zdroj: |
Reviews in Environmental Science & Biotechnology; Jun2006, Vol. 5 Issue 2/3, p233-242, 10p, 3 Diagrams, 6 Graphs |
| Abstrakt: |
In this review we explore the advantages deriving from the use of either enzymes or sugar binding proteins isolated from thermophilic organisms to develop stable fluorescence biosensors. We report on a novel approach to address the consumption of the analyte by enzyme-based biosensors, namely the utilization of apo-enzymes as non-active forms of proteins which are still able to bind the ligand but cannot transform it into product. We also report recent studies in which the fluorescence labeling of a naturally thermostable binding protein allows a quantitative determination of glucose. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Full text from SpringerLink