| Autor: |
Ania C. Thackray, Rachel L. Sammons, Lynne E. Macaskie, Ping Yong, Harriet Lugg, Peter M. Marquis |
| Zdroj: |
Journal of Materials Science: Materials in Medicine; Apr2004, Vol. 15 Issue 4, p403-406, 4p |
| Abstrakt: |
A species of Serratia bacteria produces nano-crystalline hydroxyapatite (HA) crystals by use of a cell-bound phosphatase enzyme, located both periplasmically and within extracellular polymeric materials. The enzyme functions in resting cells by cleaving glycerol-2-phosphate (G-2-P) to liberate free phosphate ions which combine with calcium in solution to produce a cell-bound calcium phosphate material. Bacteria grown as a biofilm on polyurethane reticulated foam cubes were challenged with calcium and G-2-P in a bioreactor to produce a 3-D porous bone-substitute material. The scaffold has 1 mm macropores and 1 μm micropores. XRD showed the crystallites to be 2528 nm in size, resembling HA before sintering and β-tricalcium phosphate (β-TCP, whitlockite) after. When biofilm was grown on titanium discs and challenged with calcium and G-2-P, a calcium phosphate layer formed on the discs. Biomineralisation is therefore a potential route to production of precursor nanophase HA, which has the potential to improve strength. The scaffold material produced by this method could be used as a bone-filler or as an alternative method for coating implants with a layer of HA. [ABSTRACT FROM AUTHOR] |
| Databáze: |
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